Development of novel polymorphic microsatellite markers in Siganus fuscescens
نویسندگان
چکیده
منابع مشابه
Development of novel polymorphic microsatellite markers in Siganus fuscescens.
Rabbitfish, Siganus fuscescens, is widely distributed in the Indo-Pacific regions and eastern Mediterranean. Its dwelling place includes reef flats, coral reef regions, and seagrass meadows in tropical area and reef areas or shallow waters in locations at high latitudes. In the present study, 10 new polymorphic microsatellite markers were screened from 30 wild S. fuscescens individuals, using a...
متن کاملCharacterization of new microsatellite markers of Siganus fuscescens (Siganidae).
Siganus fuscescens, which is a small commercially important marine fish, is wildly distributed in shallow waters throughout the tropical and subtropical Indo-Pacific and Eastern Mediterranean regions. It is part of a group known as rabbitfish. Fifteen new polymorphic microsatellite markers for S. fuscescens were identified, and 32 wild individuals were used to evaluate the degree of polymorphis...
متن کاملIsolation and characterization of novel microsatellite markers for molecular genetic diversity in Siganus fuscescens.
The rabbitfish Siganus fuscescens is an economically valuable species that is widely distributed throughout the estuaries, intertidal, and offshore coasts of the Indo-Pacific and eastern Mediterranean. Ten novel microsatellite loci from the genome of S. fuscescens were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats. Polymo...
متن کاملDiscovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin.
Nine microsatellite DNA markers were developed and characterized for Siganus oramin by the 5'-anchored polymerase chain reaction technique. A total of 42 alleles were identified in 30 individuals, and the number of alleles per locus ranged from 3 to 7, with an average of 4.7. The observed and expected heterozygosity per locus ranged from 0.5333 to 1.0000 and from 0.5254 to 0.8474, respectively,...
متن کاملA Novel Approach for Mining Polymorphic Microsatellite Markers In Silico
An important emerging application of high-throughput 454 sequencing is the isolation of molecular markers such as microsatellites from genomic DNA. However, few studies have developed microsatellites from cDNA despite the added potential for targeting candidate genes. Moreover, to develop microsatellites usually requires the evaluation of numerous primer pairs for polymorphism in the focal spec...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2016
ISSN: 1676-5680
DOI: 10.4238/gmr.15037515